Nanoprobes offers gold and other metal nanoparticles and related products for biomedical imaging and detection. Nanoprobes products are used for electron and light microscopy immunolabeling, research and preclinical small animal micro-CT imaging of vasculature, tumors and organs, rapid diagnostics, correlative microscopy, light microscopic immunohistochemistry (IHC) and in situ hybridization (ISH), and for Western and other types of blotting and detection.
Products include:
> Nanogold® gold nanoparticle labeling reagents. Unlike conventional immunogold probes, in which colloidal gold particles are electrostatically adsorbed to antibodies and proteins, Nanogold® is uncharged and is cross-linked to specific sites on biomolecules. Choose from maleimido- (thiol-reactive), Sulfo-NHS (amine-reactive), amino- (may be cross-linked using a variety of reagents) and Click reactivity for bioorthogonal labeling. This gives Nanogold® a range and versatility of conjugation which is not available with colloidal gold. Nanogold® can be attached to any molecule with a reactive group -- proteins, peptides, oligonucleotides, small molecules and lipids.
> Nanogold®-IgG, Fab' and Streptavidin conjugates for immunogold labeling for light and electron microscopy.
> FluoroNanogold™ IgG, Fab' and Streptavidin conjugates These probes combine both the 1.4 nm Nanogold® gold nanoparticle and a fluorescent label into a single probe for imaging a specimen both by fluorescence and electron microscopy. Use FluoroNanogold™ to check immunolabeling by fluorescence before EM processing, or to correlate target distribution at the cellular and macromolecular level.
> Gold nanomaterials: coated gold nanoparticles with a choice of different solubility characteristics.
> Silver enhancement and GoldEnhance™ reagents. These are used to amplify Nanogold® signals for microscopy and blots. They deposit silver or gold (respectively) onto gold nanoparticles, enlarging them for easier electron microscopic visualization, or rendering them visible for optical microscopy or detection.
> AuroVist™ gold nanoparticle contrast agents for micro-CT imaging in small animals in a preclinical or research setting. These provide maximum half-life in the vascular system (24 hours), together with very high contrast and contrast resolution in a non-viscous, very low toxicity formulation.
VivoVist™ is a revolutionary nanotechnology super contrast agent of alkaline earth metal nanoparticles. VivoVist™ enables greatly enhanced X-ray imaging of blood vessels, tumors, and other tissues and organs. It is particularly useful for in vivo live animal microCT imaging, for studies of tumors, stroke, atherosclerosis and other vascular conditions, organ function, and other biological structural and functional analyses.
Features and Advantages
- VivoVist™ provides approximately 3-4 times higher contrast than competing commercial microCT contrast agents. An Initial blood concentration of 50 mg/mL (5%), or 1 g/kg body weight (one-third of one vial for a 25 g mouse) gives greater than 3500 HU (Hounsfeld Units) contrast.
- Long blood half-life of 14 hrs. VivoVist™ stays in the circulatory system longer than competing products. This enables extended imaging times and data collection, and allows longer diffusion into tumors and other features of interest, increasing their contrast.
- Lowest price - makes imaging rats and larger animals affordable. VivoVist™ is more concentrated that competing products, meaning that fewer units are required to image larger animals. Less than two vials will provide good contrast in a 250 g rat.
- Low toxicity (4 g/kg is well-tolerated). VivoVist™ can be administered in higher doses than competing products, to produce super-high contrst in fine structures or to accelerate loading of tumors or other features of interest.
- Low osmolality, even at high concentrations. This means that injecting VivoVist™ will not greatly change the levels of electrolytes in the circulatory system. Impacts to metabolism, signaling and other processes that depend on maintaining a steady ionic strength are minimized.
- Low viscosity: easy to inject into small mouse tail vein blood vessels (typical injection volume 0.25 mL). VivoVist™ is more easily injected, causes less injection site trauma to the animal, and may be administered more quickly and in higher concentration that more viscous contrast agents such as iodine-based reagents.
- Can be imaged using MicroCT, clinical CT, planar X-ray, or mammography units. VivoVist™ is adaptable for any commonly used computed tomography system, giving users the flexibility to image in any desired instrument configuration.
- Enhances radiotherapy X-ray dose to tumors and other targets. Because it absorbs X-rays so strongly, VivoVist™ increases the local X-ray dose. If it is concentrated in tumors or other therapeutic targets, it can provide a method for enhancing radiotherapy and increasing the efficacy of cancer therapy.
NEW
GoldEnhance™ EM Plus - Develop with gold for the very best enhancement.
Get deep penetration with our ultra-small gold labels...Develop with GoldEnhance™ EM Plus to 3-20 nm for incredible imaging!Gold developers are better than silver, for many applications.
- Slow enhancement for an easy control of particle size
- Better for SEM: Gold gives a much better backscatter signal than silver
- Low background - No autonucleation for 40 minutes
- Neutral pH for best ultrastructural preservation
- Permanent staining: does not fade
- Safe to use before osmium tetroxide etching --silver would dissolve.
- Can be used for specimens on metal surfaces (e.g. cell culture substrates)
- Light insensitive, Low viscosity, Mix-and-use
- Our new favorite for EM!